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Prevalence of pks-positive Escherichia coli in Japanese patients with or without colorectal cancer tumors

Prevalence of pks-positive Escherichia coli in Japanese patients with or without colorectal cancer tumors

Abstract

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Background

Present tests also show that some Escherichia coli strains possessing a gene cluster known as the pks island might have a causative role in the development of human being colorectal cancer tumors (CRC). In many reports from Europe, they have been discovered more prevalently in colon tissue specimens based on CRC patients compared to those from controls. In this study we desired to explain the distinction in pks prevalence between CRC patients and non-CRC settings within the population that is japanese using non-invasive test collection technique during colonoscopy.

Techniques

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Colonic lavage samples were collected during diagnostic colonoscopy, and DNA that is bacterial each sample was extracted. Fecal DNA examples were then analyzed for pks island genes making use of main-stream qualitative PCR and real-time PCR that is quantitative. In some clients biopsy samples were additionally gathered in identical session of colonoscopy, while the correlation between the pks status associated with colonic lavage test while the biopsy sample of the identical patients had been assessed.

Outcomes

Twelve away from thirteen patients (92%) showed similar pks status by colonic lavage sample and biopsy sample, suggesting the effectiveness of colonic lavage examples as a surrogate for biopsy samples. An overall total of 98 colonic lavage samples had been gathered, which included 35 from CRC clients, 37 from adenoma clients, and 26 from controls.

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